Potassium Channel KAT3 antibody
This anti-Potassium Channel KAT3 antibody is a Rabbit Polyclonal antibody detecting Potassium Channel KAT3 in WB. Suitable for Arabidopsis thaliana.
Catalog No. ABIN488529
Quick Overview for Potassium Channel KAT3 antibody (ABIN488529)
Target
Potassium Channel KAT3 (KAT3)
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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Predicted Reactivity
- Arabidopsis thaliana
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 75.5 / 75 kDa
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Purification
- serum
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Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
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Application Notes
- 1: 50 with 125I (WB)
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Comment
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In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water.
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Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Potassium Channel KAT3 (KAT3)
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Alternative Name
- KC1
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Background
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AGI Code: At4g32650
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC -
Molecular Weight
- expected: 75.5 kDa, apparent: 75 kDa
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UniProt
- P92960
Target
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