Potassium Channel KAT3 antibody
Quick Overview for Potassium Channel KAT3 antibody (ABIN488529)
Target
Reactivity
Host
Clonality
Application
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Purpose
- Anti-KC1 | potassium channel KAT3
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Cross-Reactivity (Details)
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Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Predicted Reactivity
- Arabidopsis thaliana
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Purification
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960
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Application Notes
- 1 : 50 with 125I (WB)
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Comment
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In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Potassium Channel KAT3 (KAT3)
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Alternative Name
- KC1
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Background
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Background: KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Additional information: For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
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Molecular Weight
- 75.5 | 75 kDa
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Gene ID
- 829400
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UniProt
- P92960
Target
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