PRDX6-PS2 antibody (C-Term)
There are 2+ publications for this product available. The Rabbit Polyclonal anti-PRDX6-PS2 antibody is suitable to detect PRDX6-PS2 in samples from Arabidopsis thaliana. It has been validated for ELISA and WB.
(2 references)
Catalog No. ABIN125822
$804.80
Plus shipping costs $50.00
100 μL
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Quick Overview for PRDX6-PS2 antibody (C-Term) (ABIN125822)
Target
PRDX6-PS2
(Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
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Binding Specificity
- C-Term
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Purpose
- Anti-GPX | Glutathione peroxidase (chloroplastic)
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Cross-Reactivity (Details)
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Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Purification
- Serum
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Immunogen
- thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production
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Application Notes
- 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)
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Comment
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This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water
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Buffer
- Serum
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).
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: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
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- PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
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Alternative Name
- GPX
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Background
- GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
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Molecular Weight
- 25 | 20 kDa
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Gene ID
- 829316
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UniProt
- Q9SZ54
Target
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