ILK Protein (AA 1-452) (His tag)
Quick Overview for ILK Protein (AA 1-452) (His tag) (ABIN1000951)
Target
See all ILK ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-452
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Purification tag / Conjugate
- This ILK protein is labelled with His tag.
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Purpose
- Integrin-Linked Kinase (ILK) (Western Blot Control)
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Sequence
- MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW MIVPILEKMQ DK
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Characteristics
- recombinant ILK1 comprises a 452 amino acid fragment (1-452) corresponding to the mature ILK1 fulllength protein and is expressed in E. coli with an aminoterminal hexahistidine tag.
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Integrin-Linked Kinase (ILK) (AA 1-452) protein (His tag)
custom-made ILK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Integrin-Linked Kinase (ILK) (AA 1-452) protein (His tag)custom-made ILK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Integrin-Linked Kinase (ILK) (AA 1-452) protein (GST tag)ILK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Integrin-Linked Kinase (ILK) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ILK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Integrin-Linked Kinase (ILK) (AA 1-228), (partial) protein (His tag)ILK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied in 1x Laemmli Buffer (25 mM Tris-HCl pH 6.8, 50 mM DTT, 1 % (w/v) SDS, 0.1 % (w/v) Bromophenol Blue, 2.5 % Glycerol).
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C
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- ILK (Integrin-Linked Kinase (ILK))
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Alternative Name
- Integrin-Linked Kinase
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Background
- Integrin-linked kinase (ILK1) is a serine/threonine protein kinase, containing 4 ankyrin-like repeats. ILK1 interacts with the cytoplasmic domains of integrin β1 and β3 subunits as well as several adaptors and signalling proteins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. ILK1 has been reported to regulate a number of biological properties including anchorage-independent cell cycle progression, tumour cell invasion and apoptosis. Over-expression of ILK1 has been documented in a wide variety of human malignancies.,ILK-1, ILK-2, 59 kDa serine/threonine-protein kinase, p59, p59ILK.
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Molecular Weight
- 55.92 kDa
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UniProt
- Q13418
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Pathways
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration
Target
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