Streptavidin Protein (Atto 425)

Catalog No. ABIN1045077

Supplier Product No.: s000-51

This Native Streptavidin protein is expressed in Streptomyces avidinii.

Quick Overview for Streptavidin Protein (Atto 425) (ABIN1045077)

Target: Streptavidin

Protein Type: Native

Origin: Streptomyces avidinii

Source: Streptomyces avidinii

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA, Dot Blot (DB), Immunofluorescence (IF)

Product Details

Purification tag / Conjugate: This Streptavidin protein is labelled with Atto 425.

Supplier Product No.: s000-51

Supplier: Rockland

Purpose: Streptavidin ATTO 425 Conjugated

Purification: STREPTAVIDIN ATTO 425 was prepared from chromatographically purified Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Streptavidin. No reaction was observed against anti-Avidin.

Application Details

Application Notes:

Application Note: Streptavidin ATTO 425 has been tested by dot blot, western blot, and immunofluorescence. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 500 μL)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Streptavidin

Background:

Synonyms: SA, S avidin, streptococcus avidin, streptavidin AT425, ATTO 425, ATTO-TEC 425, STREPTAVIDIN ATTO 425 Conjugated

Background: STREPTAVIDIN ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, uti