Cytochrome b5 (CYTB5) (AA 2-97), (partial) protein (GST tag)

Catalog No. ABIN1046908

Product Details

Target: Cytochrome b5 (CYTB5)

Protein Type: Recombinant

Protein Characteristics: AA 2-97, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: GST tag

Application: ELISA

Sequence: AEQSDEAVKY YTLEEIQKHN HSKSTWLILH HKVYDLTKFL EEHPGGEEVL REQAGGDATE NFEDVGHSTD AREMSKTFII GELHPDDRPK LNKPPEP

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Li, Giordano, Yoo, Steggles: "The isolation and characterization of the human cytochrome b5 gene." in: Biochemical and biophysical research communications, Vol. 209, Issue 3, pp. 894-900, (1995) (PubMed).

Giordano, Steggles: "The human liver and reticulocyte cytochrome b5 mRNAs are products from a single gene." in: Biochemical and biophysical research communications, Vol. 178, Issue 1, pp. 38-44, (1991) (PubMed).

Yoo, Steggles: "The complete nucleotide sequence of human liver cytochrome b5 mRNA." in: Biochemical and biophysical research communications, Vol. 156, Issue 1, pp. 576-80, (1988) (PubMed).

Target Details

Target: Cytochrome b5 (CYTB5)

Alternative Name: Cytochrome b5 protein (CYTB5 Products)

Background: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Molecular Weight: 38.5 kD

UniProt: P00167