Cytochrome b5 (CYTB5) (AA 2-134) protein (His tag)

Catalog No. ABIN1096509

This Recombinant Cytochrome b5 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Cytochrome b5 (CYTB5) (AA 2-134) protein (His tag) (ABIN1096509)

Target: Cytochrome b5 (CYTB5)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-134

Purification tag / Conjugate: His tag

Purpose: Recombinant Human Cytochrome b5/CYB5A (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAEQSDEAVK YYTLEEIQKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT ENFEDVGHST DAREMSKTFI IGELHPDDRP KLNKPPETLI TTIDSSSSWW TNWVIPAISA VAVALMYRLY MAED

Characteristics: Recombinant Human Cytochrome b5/CYB5A is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Asp134) of Human CYB5A fused with a His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 0.1 mM EDTA, pH 7.25.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Cytochrome b5 (CYTB5)

Alternative Name: Cytochrome-b5

Sub Type: Fusionprotein

Background: Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.
Alternative Names: Cytochrome b5, Microsomal Cytochrome b5 Type A, MCB5, CYB5A, CYB5

Molecular Weight: 17.5 kDa

UniProt: P00167