UBE2I Protein (AA 1-157, partial) (GST tag)

Catalog No. ABIN1095762

Product Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Protein Type: Recombinant

Protein Characteristics: AA 1-157, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2I protein is labelled with GST tag.

Application: ELISA

Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAP

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Tachibana, Iwata, Watanabe, Nobukuni, Ploplis, Kajigaya: "Assignment of the gene for a ubiquitin-conjugating enzyme (UBE2I) to human chromosome band 16p13.3 by in situ hybridization." in: Cytogenetics and cell genetics, Vol. 75, Issue 4, pp. 222-3, (1997) (PubMed).

Watanabe, Fujiwara, Kawai, Shimizu, Takami, Hirano, Okuno, Ozaki, Takeda, Shimada, Nagata, Takaichi, Takahashi, Nakamura, Shin: "Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle." in: Cytogenetics and cell genetics, Vol. 72, Issue 1, pp. 86-9, (1996) (PubMed).

Yasugi, Howley: "Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9." in: Nucleic acids research, Vol. 24, Issue 11, pp. 2005-10, (1996) (PubMed).

Target Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: SUMO-conjugating enzyme UBC9 protein (UBE2I Products)

Synonyms: C358B7.1 Protein, P18 Protein, UBC9 Protein, ubc9 Protein, ube2ia Protein, zUbc9 Protein, 5830467E05Rik Protein, F830028O17Rik Protein, Ubce2i Protein, Ubce9 Protein, UbcE2A Protein, ubce9 Protein, T13J8.70 Protein, T13J8_70 Protein, UBIQUITIN-PROTEIN LIGASE Protein, ubiquitin conjugating enzyme 9 Protein, ubiquitin conjugating enzyme E2 I Protein, ubiquitin-conjugating enzyme E2Ib Protein, ubiquitin-conjugating enzyme E2L Protein, ubiquitin-conjugating enzyme E2I Protein, ubiquitin conjugating enzyme E2I S homeolog Protein, ubiquitin conjugating enzyme 9 Protein, SUMO-conjugating enzyme UBC9 Protein, UBE2I Protein, ube2ib Protein, UBE2L Protein, Ube2i Protein, ube2i.S Protein, UBC9 Protein, ubc-9 Protein, LOC108703134 Protein

Background: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.

Molecular Weight: 45.3 kD

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway