TLN1 Protein (AA 92-399, partial) (GST tag)

Catalog No. ABIN1047720

This Recombinant TLN1 protein is produced in Escherichia coli (E. coli). There are 2 publications available.

Quick Overview for TLN1 Protein (AA 92-399, partial) (GST tag) (ABIN1047720)

Target: TLN1 (Talin 1 (TLN1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 92-399, partial

Purification tag / Conjugate: This TLN1 protein is labelled with GST tag.

Sequence: MLDGTVKTIM VDDSKTVTDM LMTICARIGI TNHDEYSLVR ELMEEKKEEI TGTLRKDKTL LRDEKKMEKL KQKLHTDDEL NWLDHGRTLR EQGVEEHETL LLRRKFFYSD QNVDSRDPVQ LNLLYVQARD DILNGSHPVS FDKACEFAGF QCQIQFGPHN EQKHKAGFLD LKDFLPKEYV KQKGERKIFQ AHKNCGQMSE IEAKVRYVKL ARSLKTYGVS FFLVKEKMKG KNKLVPRLLG ITKECVMRVD EKTKEVIQEW NLTNIKRWAA SPKSFTLDFG DYQDGYYSVQ TTEGEQIAQL IAGYIDII

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Nakajima, Okazaki, Yamakawa, Kikuno, Ohara, Nagase: "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 9, Issue 3, pp. 99-106, (2002) (PubMed).

Ben-Yosef, Francomano: "Characterization of the human talin (TLN) gene: genomic structure, chromosomal localization, and expression pattern." in: Genomics, Vol. 62, Issue 2, pp. 316-9, (2000) (PubMed).

Target Details

Target: TLN1 (Talin 1 (TLN1))

Alternative Name: Talin-1 protein

Background: Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts By similarity.

Molecular Weight: 63.2 kD

UniProt: Q9Y490

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location