Annexin V Protein (DY-490)
(1 reference)Quick Overview for Annexin V Protein (DY-490) (ABIN1047953)
Target
See all Annexin V (ANXA5) ProteinsProtein Type
Origin
Host
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Purification tag / Conjugate
- This Annexin V protein is labelled with DY-490.
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Characteristics
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Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5 -
Material not included
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Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml
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Annexin A5 (ANXA5) (AA 2-320) protein
ANXA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Annexin A5 (ANXA5) protein (FITC)ANXA5 Origin: Human Host: Bacteria Recombinant > 99 % (SDS-PAGE) FACS
Annexin A5 (ANXA5) (AA 1-319) protein (His-EGFP Tag)ANXA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Annexin A5 (ANXA5) (AA 1-320) proteinANXA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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Application Notes
- 5 l are suitable for one assay
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Assay Procedure
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Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour). -
Restrictions
- For Research Use only
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Format
- Liquid
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
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: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
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