ANG Protein (AA 25-147)

Catalog No. ABIN1096005

This Recombinant ANG protein is expressed in Escherichia coli (E. coli).

Quick Overview for ANG Protein (AA 25-147) (ABIN1096005)

Target: ANG (Angiogenin (ANG))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 25-147

Purpose: Recombinant Human Angiogenin/ANG/RNASE5

Sequence: MQDNSRYTHF LTQHYDAKPQ GRDDRYCESI MRRRGLTSPC KDINTFIHGN KRSIKAICEN KNGNPHRENL RISKSSFQVT TCKLHGGSPW PPCQYRATAG FRNVVVACEN GLPVHLDQSI FRRP

Characteristics: Recombinant Human Angiogenin is produced by our E. coli expression system. The target protein is expressed with sequence (Gln25-Pro147) of Human Angiogenin.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: ANG (Angiogenin (ANG))

Alternative Name: angiogenin

Background: Angiogenin belongs to the pancreatic ribonuclease family. Angiogenin is primarily expressed in the liver. It may act as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Angiogenin is a potent stimulator of new blood vessel formation. And Angiogenin is endocytosed and translocated to the nucleus by binding to actin on the surface of endothelial cells. Angiogenic activity is regulated by interaction with RNH1 in vivo. In addition, Angiogenin is associated with susceptibility to amyotrophic lateral sclerosis, which is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord.
Alternative Names: Angiogenin, Ribonuclease 5, RNase 5, ANG, RNASE5

Molecular Weight: 14.27 kDa

UniProt: P03950