AIF Protein (AA 121-613) (His tag)
Quick Overview for AIF Protein (AA 121-613) (His tag) (ABIN1096041)
Target
See all AIF (AIFM1) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 121-613
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Purification tag / Conjugate
- This AIF protein is labelled with His tag.
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Purpose
- Recombinant Human Apoptosis-Inducing Factor 1 Mitochondrial/AIFM1 (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MEEVPQDKAP SHVPFLLIGG GTAAFAAARS IRARDPGARV LIVSEDPELP YMRPPLSKEL WFSDDPNVTK TLRFKQWNGK ERSIYFQPPS FYVSAQDLPH IENGGVAVLT GKKVVQLDVR DNMVKLNDGS QITYEKCLIA TGGTPRSLSA IDRAGAEVKS RTTLFRKIGD FRSLEKISRE VKSITIIGGG FLGSELACAL GRKARALGTE VIQLFPEKGN MGKILPEYLS NWTMEKVRRE GVKVMPNAIV QSVGVSSGKL LIKLKDGRKV ETDHIVAAVG LEPNVELAKT GGLEIDSDFG GFRVNAELQA RSNIWVAGDA ACFYDIKLGR RRVEHHDHAV VSGRLAGENM TGAAKPYWHQ SMFWSDLGPD VGYEAIGLVD SSLPTVGVFA KATAQDNPKS ATEQSGTGIR SESETESEAS EITIPPSTPA VPQAPVQGED YGKGVIFYLR DKVVVGIVLW NIFNRMPIAR KIIKDGEQHE DLNEVAKLFN IHED
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Characteristics
- Recombinant Human Apoptosis-Inducing Factor 1, Mitochondrial/AIFM1 is produced by our E. coli expression system. The target protein is expressed with sequence (Glu121-Asp613) of Human AIFM1 fused with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
Expiry Date
- 3 months
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- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
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Alternative Name
- AIF
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Sub Type
- Fusionprotein
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Background
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Apoptosis-Inducing Factor 1, Mitochondrial (AIFM1) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. During apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces parthanatos i.e., caspase-independent fragmentation of chromosomal DNA. AIFM1 interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. It binds to DNA in a sequence-independent manner and plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells.ReferencesTezel G, et al. Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.PMID: 23150628 [PubMed - indexed for MEDLINE] PMCID: PMC3522442 http://www.ncbi.nlm.nih.gov/pubmed/23150628
Alternative Names: Apoptosis-Inducing Factor 1 Mitochondrial, Programmed Cell Death Protein 8, AIFM1, AIF, PDCD8 -
Molecular Weight
- 56.2 kDa
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UniProt
- O95831
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Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
Target
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