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AIF Protein (AA 121-613) (His tag)

This Recombinant AIF protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN1096041

Quick Overview for AIF Protein (AA 121-613) (His tag) (ABIN1096041)

Target

See all AIF (AIFM1) Proteins
AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Protein Type

Recombinant

Origin

  • 10
  • 4
Human

Source

  • 10
  • 2
  • 1
  • 1
Escherichia coli (E. coli)

Purity

> 95 % as determined by reducing SDS-PAGE.
  • Protein Characteristics

    AA 121-613

    Purification tag / Conjugate

    This AIF protein is labelled with His tag.

    Purpose

    Recombinant Human Apoptosis-Inducing Factor 1 Mitochondrial/AIFM1 (N-6His)

    Sequence

    MGSSHHHHHH SSGLVPRGSH MEEVPQDKAP SHVPFLLIGG GTAAFAAARS IRARDPGARV LIVSEDPELP YMRPPLSKEL WFSDDPNVTK TLRFKQWNGK ERSIYFQPPS FYVSAQDLPH IENGGVAVLT GKKVVQLDVR DNMVKLNDGS QITYEKCLIA TGGTPRSLSA IDRAGAEVKS RTTLFRKIGD FRSLEKISRE VKSITIIGGG FLGSELACAL GRKARALGTE VIQLFPEKGN MGKILPEYLS NWTMEKVRRE GVKVMPNAIV QSVGVSSGKL LIKLKDGRKV ETDHIVAAVG LEPNVELAKT GGLEIDSDFG GFRVNAELQA RSNIWVAGDA ACFYDIKLGR RRVEHHDHAV VSGRLAGENM TGAAKPYWHQ SMFWSDLGPD VGYEAIGLVD SSLPTVGVFA KATAQDNPKS ATEQSGTGIR SESETESEAS EITIPPSTPA VPQAPVQGED YGKGVIFYLR DKVVVGIVLW NIFNRMPIAR KIIKDGEQHE DLNEVAKLFN IHED

    Characteristics

    Recombinant Human Apoptosis-Inducing Factor 1, Mitochondrial/AIFM1 is produced by our E. coli expression system. The target protein is expressed with sequence (Glu121-Asp613) of Human AIFM1 fused with a 6His tag at the N-terminus.

    Sterility

    0.2 μm filtered

    Endotoxin Level

    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    Buffer

    Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

    Handling Advice

    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

    Storage

    4 °C/-20 °C/-80 °C

    Storage Comment

    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.

    Expiry Date

    3 months
  • Target

    AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

    Alternative Name

    AIF

    Sub Type

    Fusionprotein

    Background

    Apoptosis-Inducing Factor 1, Mitochondrial (AIFM1) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. During apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces parthanatos i.e., caspase-independent fragmentation of chromosomal DNA. AIFM1 interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. It binds to DNA in a sequence-independent manner and plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells.ReferencesTezel G, et al. Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.PMID: 23150628 [PubMed - indexed for MEDLINE] PMCID: PMC3522442 http://www.ncbi.nlm.nih.gov/pubmed/23150628
    Alternative Names: Apoptosis-Inducing Factor 1 Mitochondrial, Programmed Cell Death Protein 8, AIFM1, AIF, PDCD8

    Molecular Weight

    56.2 kDa

    UniProt

    O95831

    Pathways

    Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
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