BMP2 Protein (AA 283-396)

Catalog No. ABIN1096140

Product Details

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 283-396

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purpose: Recombinant Human Bone Morphogenetic Protein 2/BMP-2

Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Characteristics: Recombinant Human Bone Morphogenetic Protein 2/BMP-2 is produced by our E. coli expression system. The target protein is expressed with sequence (Gln283-Arg396) of Human BMP-2.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: Recombinant BMP-2 is fully biologically active when compared to standards.ED50 is less than 50 ng/ml as determined by the cytolysis of MC3T3-E1 cells.Specific Activity of 2.0 x 104 IU/mg

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in 50 mM Acetic acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM HAc .

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: bmp-2 (BMP2 Products)

Background: Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.ReferencesWu T, et al. miR-30 family members negatively regulate osteoblast differentiation.PMID: 22253433, PMCID: PMC3293535http://www.ncbi.nlm.nih.gov/pubmed/22253433
Alternative Names: Bone Morphogenetic Protein 2, BMP-2, Bone Morphogenetic Protein 2A, BMP-2A, BMP2, BMP2A

Molecular Weight: 13.3 kDa

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation