CA10 Protein (AA 22-300) (His tag)
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- Target See all CA10 Proteins
- CA10 (Carbonic Anhydrase X (CA10))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-300
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CA10 protein is labelled with His tag.
- Purpose
- Recombinant Human Carbonic Anhydrase 10/CA10 (C-6His)
- Sequence
- QQNSPKIHEG WWAYKEVVQG SFVPVPSFWG LVNSAWNLCS VGKRQSPVNI ETSHMIFDPF LTPLRINTGG RKVSGTMYNT GRHVSLRLDK EHLVNISGGP MTYSHRLEEI RLHFGSEDSQ GSEHLLNGQA FSGEVQLIHY NHELYTNVTE AAKSPNGLVV VSIFIKVSDS SNPFLNRMLN RDTITRITYK NDAYLLQGLN IEELYPETSS FITYDGSMTI PPCYETASWI IMNKPVYITR MQMHSLRLLS QNQPSQIFLS MSDNFRPVQP LNNRCIRTNV DHHHHHH
- Characteristics
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Recombinant Human Carbonic Anhydrase 10/CA10 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CA10 Protein
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Carbonic Anhydrase X (CA10) (AA 1-328) protein (His tag)
custom-made CA10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Carbonic Anhydrase X (CA10) (AA 1-328) protein (His tag)custom-made CA10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Carbonic Anhydrase X (CA10) (Active) protein (His tag)CA10 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CA10 (Carbonic Anhydrase X (CA10))
- Alternative Name
- Carbonic Anhydrase 10 (CA10 Products)
- Background
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Recombinant Human Carbonic Anhydrase-Related Protein 10/CA10 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Q22-N300) of Human CA10 fused with a polyhistidine tag at the C-terminus.
Carbonic Anhydrase X (CA10) belongs to CA family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. While CA10 is a secreted protein without Carbonic Anhydrase activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc binding site, it has esterase activity. The human and mouse CA10 are expressed in the brain, indicating that they may play a role in brain development. - Molecular Weight
- 32.82 kDa
- UniProt
- Q9NS85
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