CA10 Protein

Catalog No. ABIN7317797

This Recombinant CA10 protein is produced in HEK-293 Cells.

Quick Overview for CA10 Protein (ABIN7317797)

Target: CA10 (Carbonic Anhydrase X (CA10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human Carbonic Anhydrase 10/CA10 Protein

Sequence: Met 1-Asn 300

Characteristics: The mature form of human CARPX (NP_001076002.1) (Met 1-Asn 300) with five amino acids (DDDDK) at the C-terminus was expressed and purified.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its esterase activity. The specific activity is > 100 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CA10 (Carbonic Anhydrase X (CA10))

Alternative Name: Carbonic Anhydrase 10/CA10

Background: CA-RP X,CA-RPX,CA10,CARP X,CARPX,Carbonic Anhydrase-Related Protein 10,Carbonic Anhydrase-Related Protein X,Cerebral Protein 15,HUCEP-15,Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.

Molecular Weight:

Calculated MW: 27.2 kDa

Observed MW: 38 kDa

Gene ID: 56934

NCBI Accession: NP_001076002

UniProt: Q9NS85