Carboxypeptidase A2 Protein (AA 17-417) (His tag)

Catalog No. ABIN1096222

This Recombinant Carboxypeptidase A2 protein is produced in Human Cells.

Quick Overview for Carboxypeptidase A2 Protein (AA 17-417) (His tag) (ABIN1096222)

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 17-417

Purification tag / Conjugate: This Carboxypeptidase A2 protein is labelled with His tag.

Purpose: Recombinant Human Carboxypeptidase A2/CPA2 (C-6His)

Sequence: LETFVGDQVL EIVPSNEEQI KNLLQLEAQE HLQLDFWKSP TTPGETAHVR VPFVNVQAVK VFLGSQGIAY SIMIEDVQVL LDKENEEMLF NRRRERSGNF NFGAYHTLEE ISQEMDNLVA EHPGLVSKVN IGSSFENRPM NVLKFSTGGD KPAIWLDAGI HAREWVTQAT ALWTANKIVS DYGKDPSITS ILDALDIFLL PVTNPDGYVF SQTKNRMWRK TRSKVSGSLC VGVDPNRNWD AGFGGPGASS NPCSDSYHGP SANSEVEVKS IVDFIKSHGK VKAFITLHSY SQLLMFPYGY KCTKLDDFDE LSEVAQKAAQ SLRSLHGTKY KVGPICSVIY QASGGSIDWS YDYGIKYSFA FELRDTGRYG FLLPARQILP TAEETWLGLK AIMEHVRDHP YVDHHHHHH

Characteristics:

Recombinant Human Carboxypeptidase A2/CPA2 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150mm NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Alternative Name: Carboxypeptidase A2/CPA2

Background: Recombinant Human Carboxypeptidase A2/CPA2 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser115-Tyr417) of Human CPA2 fused with a polyhistidine tag at the C-terminus.
Carboxypeptidase A2 (CPA) is a secreted pancreatic procarboxy-peptidase that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic AAs in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition.

Molecular Weight: 45.91 kDa

UniProt: P48052