Coq7 Protein (AA 37-217) (His tag)

Catalog No. ABIN1096428

This Recombinant Coq7 protein is expressed in Human Cells.

Quick Overview for Coq7 Protein (AA 37-217) (His tag) (ABIN1096428)

Target: Coq7 (Coenzyme Q Biosynthesis Protein 7 (Coq7))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 37-217

Purification tag / Conjugate: This Coq7 protein is labelled with His tag.

Purpose: Recombinant Human 5-Demethoxyubiquinone Hydroxylase, Mitochondrial/COQ7 (C-6His)

Sequence: SGMTLDNISR AAVDRIIRVD HAGEYGANRI YAGQMAVLGR TSVGPVIQKM WDQEKDHLKK FNELMVTFRV RPTVLMPLWN VLGFALGAGT ALLGKEGAMA CTVAVEESIA HHYNNQIRTL MEEDPEKYEE LLQLIKKFRD EELEHHDIGL DHDAELAPAY AVLKSIIQAG CRVAIYLSER LVDHHHHHH

Characteristics: Recombinant Human Ubiquinone Biosynthesis Protein COQ7 Homolog/COQ7 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser37-Leu217) of Human COQ7 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Coq7 (Coenzyme Q Biosynthesis Protein 7 (Coq7))

Alternative Name: coq7

Sub Type: Fusionprotein

Background: Ubiquinone Biosynthesis Protein COQ7 Homolog (COQ7) is a mitochondrion inner membrane and peripheral membrane protein which belongs to the COQ7 family. It is expressed dominantly in heart and skeletal muscle. COQ7 is synthesized as a preprotein that is imported into the mitochondrial matrix, where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane. COQ7 is involved in lifespan determination in ubiquinone-independent manner and also involved in ubiquinone biosynthesis. COQ7 is potential central metabolic regulator. Human COQ7 protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has been shown that mutations in the gene are associated with increased life span. Defects of the gene slow down a variety of developmental and physiological processes, including the cell cycle, embryogenesis, post-embryonic growth, rhythmic behaviors and aging.
Alternative Names: Ubiquinone Biosynthesis Protein COQ7 Homolog, Coenzyme Q Biosynthesis Protein 7 Homolog, Timing Protein Clk-1 Homolog, COQ7

Molecular Weight: 21.3 kDa

UniProt: Q99807

Pathways: Tube Formation