CXCL10 Protein (AA 22-98)

Catalog No. ABIN1096457

This Recombinant CXCL10 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CXCL10 Protein (AA 22-98) (ABIN1096457)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 22-98

Purpose: Recombinant Human C-X-C Motif Chemokine 10/CXCL10

Sequence: VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP

Characteristics: Recombinant Human C-X-C Motif Chemokine Ligand 10/CXCL10 is produced by E. coli transformed with a plasmid containing sequence (Val22-Pro98) of Human CXCL10.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: ED50 is 0.02-0.06 µg/mL as determined by the ability of Recombinant CXCL10 to chemoattract human CXCR3 transfected BaF3 mouse proB cells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: ip-10

Background: Human C-X-C Motif Chemokine Ligand 10 (CXCL10) is a non-ELR chemokine secreted by various cell types, such as monocytes, endothelial cells and fibroblasts, in response to IFN-gamma. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles, such as chemoattraction for activated T-lymphocytes, inhibition of angiogenesis, and antitumor activity.
Alternative Names: C-X-C Motif Chemokine 10, 10 kDa Interferon Gamma-Induced Protein, Gamma-IP10, IP-10, Small-Inducible Cytokine B10, CXCL10, INP10, SCYB10

Molecular Weight: 8.6 kDa

UniProt: P02778