DHPS Protein (AA 1-369) (His tag)
Quick Overview for DHPS Protein (AA 1-369) (His tag) (ABIN1096524)
Target
See all DHPS ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-369
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Purification tag / Conjugate
- This DHPS protein is labelled with His tag.
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Purpose
- Recombinant Human Deoxyhypusine Synthase/DHS (C-6His)
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Sequence
- MEGSLEREAP AGALAAVLKH SSTLPPESTQ VRGYDFNRGV NYRALLEAFG TTGFQATNFG RAVQQVNAMI EKKLEPLSQD EDQHADLTQS RRPLTSCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE NGINRIGNLL VPNENYCKFE DWLMPILDQM VMEQNTEGVK WTPSKMIARL GKEINNPESV YYWAQKNHIP VFSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKCTGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RVDAQPVKVY ADASLVFPLL VAETFAQKMD AFMHEKNEDL EHHHHHH
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Characteristics
- Recombinant Human Deoxyhypusine Synthase/DHS is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp369) of Human Deoxyhypusine Synthase.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Deoxyhypusine Synthase (DHPS) (AA 1-369) protein (His tag)
custom-made DHPS Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deoxyhypusine Synthase (DHPS) (AA 1-369) protein (His tag)custom-made DHPS Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deoxyhypusine Synthase (DHPS) (AA 1-369) protein (GST tag)DHPS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Deoxyhypusine Synthase (DHPS) (Transcript Variant 1) proteinDHPS Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 20 % Glycerol, pH 8.0.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- DHPS (Deoxyhypusine Synthase (DHPS))
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Alternative Name
- Deoxyhypusine Synthase/DHS
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Background
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Human Deoxyhypusine Synthase (DHS) is vital for the first step of hypusine biosynthesis. DHS catalyzes the NAD-dependent oxidative cleavage of spermidine, the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
Alternative Names: Deoxyhypusine Synthase, DHS, DHPS, DS -
Molecular Weight
- 42.04 kDa
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UniProt
- P49366
Target
-
