EIF1B Protein (AA 1-113) (His tag)

Catalog No. ABIN1096558

Recombinant EIF1B protein expressed in Escherichia coli (E. coli).

Quick Overview for EIF1B Protein (AA 1-113) (His tag) (ABIN1096558)

Target: EIF1B (Eukaryotic Translation Initiation Factor 1B (EIF1B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-113

Purification tag / Conjugate: This EIF1B protein is labelled with His tag.

Purpose: Recombinant Human Eukaryotic Translation Initiation Factor 1B/EIF1B (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF

Characteristics: Recombinant Human Eukaryotic Translation Initiation Factor 1B/EIF1B is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Phe113) of Human EIF1B fused with a His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 500 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: EIF1B (Eukaryotic Translation Initiation Factor 1B (EIF1B))

Alternative Name: EIF1B

Sub Type: Fusionprotein

Background: Eukaryotic Translation Initiation Factor 1B (EIF1B) is an element of a complex involved in recognition of the initiator codon during the scanning process. Translation is also initiated by the function of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. EIF1B enables 43S ribosomal complexes to distinguish between cognate and near-cognate initiation codons, perceiving the nucleotide content of initiation codons.
Alternative Names: Eukaryotic Translation Initiation Factor 1b, eIF1b, Protein Translation Factor SUI1 Homolog GC20, EIF1B

Molecular Weight: 15 kDa

UniProt: O60739