GFAP Protein (AA 292-432) (His tag)

Catalog No. ABIN1096729

Recombinant GFAP protein expressed in Escherichia coli (E. coli).

Quick Overview for GFAP Protein (AA 292-432) (His tag) (ABIN1096729)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 292-432

Purification tag / Conjugate: This GFAP protein is labelled with His tag.

Purpose: Recombinant Human Glial Fibrillary Acidic Protein/GFAP (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MLTCDLESLR GTNESLERQM REQEERHVRE AASYQEALAR LEEEGQSLKD EMARHLQEYQ DLLNVKLALD IEIATYRKLL EGEENRITIP VQTFSNLQIR ETSLDTKSVS EGHLKRNIVV KTVEMRDGEV IKESKQEHKD VM

Characteristics: Recombinant Human Glial Fibrillary Acidic Protein/GFAP is produced by our E. coli expression system. The target protein is expressed with sequence (Leu292-Met432) of Human GFAP fused with a His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.25.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Sub Type: Fusionprotein

Background: Glial Fibrillary Acidic Protein (GFAP) is an intermediate filament (IF) protein which belongs to the intermediate filament family. GFAP is expressed in numerous cell types of the central nervous system (CNS), ependymal cells and phosphorylated by PKN1. GFAP, a class-III intermediate filament, is a cell-specific marker during the development of the central nervous system and distinguishes astrocytes from other glial cells. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood.
Alternative Names: Glial Fibrillary Acidic Protein, GFAP

Molecular Weight: 18.7 kDa

UniProt: P14136