Glutathione Synthetase Protein (GSS) (AA 2-474) (His tag)

Catalog No. ABIN1096753

Recombinant Glutathione Synthetase protein expressed in Escherichia coli (E. coli).

Quick Overview for Glutathione Synthetase Protein (GSS) (AA 2-474) (His tag) (ABIN1096753)

Target: Glutathione Synthetase (GSS)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-474

Purification tag / Conjugate: This Glutathione Synthetase protein is labelled with His tag.

Purpose: Recombinant Human Glutathione Synthetase/GSH Synthetase (C-6His)

Sequence: ATNWGSLLQD KQQLEELARQ AVDRALAEGV LLRTSQEPTS SEVVSYAPFT LFPSLVPSAL LEQAYAVQMD FNLLVDAVSQ NAAFLEQTLS STIKQDDFTA RLFDIHKQVL KEGIAQTVFL GLNRSDYMFQ RSADGSPALK QIEINTISAS FGGLASRTPA VHRHVLSVLS KTKEAGKILS NNPSKGLALG IAKAWELYGS PNALVLLIAQ EKERNIFDQR AIENELLARN IHVIRRTFED ISEKGSLDQD RRLFVDGQEI AVVYFRDGYM PRQYSLQNWE ARLLLERSHA AKCPDIATQL AGTKKVQQEL SRPGMLEMLL PGQPEAVARL RATFAGLYSL DVGEEGDQAI AEALAAPSRF VLKPQREGGG NNLYGEEMVQ ALKQLKDSEE RASYILMEKI EPEPFENCLL RPGSPARVVQ CISELGIFGV YVRQEKTLVM NKHVGHLLRT KAIEHADGGV AAGVAVLDNP YPVLEHHHHH H

Characteristics: Recombinant Human Glutathione Synthetase/GSS is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Val474) of Human GSS fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, pH 7.5 .

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Glutathione Synthetase (GSS)

Alternative Name: GSH-synthetase

Sub Type: Fusionprotein

Background: Glutathione Synthetase belongs to the eukaryotic GSH synthase family. Glutathione Synthetase is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine to form glutathione. Glutathione play an important role in a variety of biological functions, including detoxification of xenobiotics, protection of cells from oxidative damage by free radicals, and membrane transport. The protein functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in Glutathione Synthetase can also cause the glutathione synthetase deficiency of erythrocytes, which is a mild form causing hemolytic anemia.
Alternative Names: Glutathione Synthetase, GSH Synthetase, GSH-S, Glutathione Synthase, GSS

Molecular Weight: 53.45 kDa

UniProt: P48637

Pathways: Warburg Effect