HPRT1 Protein (AA 2-218) (His tag)

Catalog No. ABIN1096819

Recombinant HPRT1 protein expressed in Escherichia coli (E. coli).

Quick Overview for HPRT1 Protein (AA 2-218) (His tag) (ABIN1096819)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-218

Purification tag / Conjugate: This HPRT1 protein is labelled with His tag.

Purpose: Recombinant Human HGPRT/HPRT1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKAVE HHHHHH

Characteristics: Recombinant Human Hypoxanthine-Guanine Phosphoribosyltransferase/HGPRT is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Ala218) of Human HPRT1 fused with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 250 mM NaCl, 2 mM EDTA, 30 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HGPRTase

Sub Type: Fusionprotein

Background: Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT) has an important role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 functions to salvage purines from degraded DNA to renewed purine synthesis, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
Alternative Names: Hypoxanthine-Guanine Phosphoribosyltransferase, HGPRT, HGPRTase, HPRT1, HPRT

Molecular Weight: 27.79 kDa

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process