HPRT1 Protein (AA 1-218) (His tag)
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- Target See all HPRT1 Proteins
- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-218
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HPRT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate(GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
- Top Product
- Discover our top product HPRT1 Protein
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Hypoxanthine phosphoribosyltransferase 1 (HPRT1) (AA 1-218) protein (GST tag)
HPRT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) (AA 2-218) protein (His tag)Crystallography grade HPRT1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) protein (Myc-DYKDDDDK Tag)HPRT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing, 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
- Alternative Name
- HPRT (HPRT1 Products)
- Synonyms
- HGPRT Protein, HPRT Protein, C81579 Protein, HPGRT Protein, Hprt1 Protein, Hgprtase Protein, Hprt Protein, id:ibd1344 Protein, id:ibd5108 Protein, wu:fc10g09 Protein, zgc:56221 Protein, zgc:86608 Protein, hgprt Protein, hprt Protein, prtfdc1 Protein, hprt1 Protein, hprt1l Protein, zgc:55561 Protein, zgc:86771 Protein, hypoxanthine phosphoribosyltransferase 1 Protein, hypoxanthine guanine phosphoribosyl transferase Protein, hypoxanthine phosphoribosyltransferase 1 L homeolog Protein, phosphoribosyl transferase domain containing 1 Protein, HPRT1 Protein, Hprt Protein, Hprt1 Protein, hprt1 Protein, hprt1.L Protein, prtfdc1 Protein
- Background
- Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 26.7 kDa (238aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_000185
- UniProt
- P00492
- Pathways
- Ribonucleoside Biosynthetic Process
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