HAO1 Protein (AA 1-370) (His tag)

Catalog No. ABIN1096843

Recombinant HAO1 protein expressed in Escherichia coli (E. coli).

Quick Overview for HAO1 Protein (AA 1-370) (His tag) (ABIN1096843)

Target: HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-370

Purification tag / Conjugate: This HAO1 protein is labelled with His tag.

Purpose: Recombinant Human Hydroxyacid Oxidase 1/HAO1 (N-Trx, 6His)

Sequence: MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM ADIGSMLPRL ICINDYEQHA KSVLPKSIYD YYRSGANDEE TLADNIAAFS RWKLYPRMLR NVAETDLSTS VLGQRVSMPI CVGATAMQRM AHVDGELATV RACQSLGTGM MLSSWATSSI EEVAEAGPEA LRWLQLYIYK DREVTKKLVR QAEKMGYKAI FVTVDTPYLG NRLDDVRNRF KLPPQLRMKN FETSTLSFSP EENFGDDSGL AAYVAKAIDP SISWEDIKWL RRLTSLPIVA KGILRGDDAR EAVKHGLNGI LVSNHGARQL DGVPATIDVL PEIVEAVEGK VEVFLDGGVR KGTDVLKALA LGAKAVFVGR PIVWGLAFQG EKGVQDVLEI LKEEFRLAMA LSGCQNVKVI DKTLVRKNPL AVSKI

Characteristics: Recombinant Human Hydroxyacid Oxidase 1/HAO1 isproduced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ile370) of Human HAO1 fused with a TraxA/His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))

Alternative Name: HAO1

Sub Type: Fusionprotein

Background: Hydroxyacid Oxidase 1 (HAO1) is an enzyme that belongs to the FMN-Dependent alpha-Hydroxy Acid Dehydrogenase family. HAO1 contains 1 FMN Hydroxy Ccid Dehydrogenase domain. HAO1 is expressed primarily in the liver and pancreas. This protein has 2-Hydroxyacid Oxidase activity. Most HAO1 is active on the 2-Carbon substrate Glycolate, but it can also be active on 2-Hydroxy fatty acids, with higher activity towards 2-Hydroxy Palmitate and 2-Hydroxy Octanoate.
Alternative Names: Hydroxyacid Oxidase 1, HAOX1, Glycolate Oxidase, GOX, HAO1, GOX1, HAOX1

Molecular Weight: 58.6 kDa

UniProt: Q9UJM8

Pathways: Monocarboxylic Acid Catabolic Process