LUM Protein (AA 19-338) (His tag)

Catalog No. ABIN1097110

Recombinant LUM protein expressed in Human Cells.

Quick Overview for LUM Protein (AA 19-338) (His tag) (ABIN1097110)

Target: LUM (Lumican (LUM))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-338

Purification tag / Conjugate: This LUM protein is labelled with His tag.

Purpose: Recombinant Human Lumican/LUM (C-6His)

Sequence: QYYDYDFPLS IYGQSSPNCA PECNCPESYP SAMYCDELKL KSVPMVPPGI KYLYLRNNQI DHIDEKAFEN VTDLQWLILD HNLLENSKIK GRVFSKLKQL KKLHINHNNL TESVGPLPKS LEDLQLTHNK ITKLGSFEGL VNLTFIHLQH NRLKEDAVSA AFKGLKSLEY LDLSFNQIAR LPSGLPVSLL TLYLDNNKIS NIPDEYFKRF NALQYLRLSH NELADSGIPG NSFNVSSLVE LDLSYNKLKN IPTVNENLEN YYLEVNQLEK FDIKSFCKIL GPLSYSKIKH LRLDGNRISE TSLPPDMYEC LRVANEVTLN VDHHHHHH

Characteristics: Recombinant Human Lumican/LUM produced by transfected human cells is a secreted protein with sequence (Gln19-Asn338) of Human Lumican fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM EDTA, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: LUM (Lumican (LUM))

Alternative Name: lumican

Sub Type: Fusionprotein

Background: Lumican is a 40 kD secreted protein which belongs to the small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily. Human Lumican is synthesized as a 338 amino acid precursor then cut the 18 aa signal sequence. The mature Human Lumican contains 12 leucine-rich repeats (LRRs), 4 potential sites of N-linked glycosylation, and a C- terminal with two conserved cyst-eines. Lumican can be existed in extracellular matrix of human articular cartilage. Lumican participates in the maintenance of tissue homeostasis and regulates cellular functions in vivo, such as cell proliferation, adhesion, migration, and differentiation. The overexpression of lumican has been correlated to colorectal tumor, breast, neuroendocrine, and pancreatic cancers.
Alternative Names: Lumican, Keratan Sulfate Proteoglycan Lumican, KSPG Lumican, LUM, LDC, SLRR2D

Molecular Weight: 37.7 kDa

UniProt: P51884

Pathways: Glycosaminoglycan Metabolic Process