LUM Protein (AA 1-338) (GST tag)
(2 references)-
- Target See all LUM Proteins
- LUM (Lumican (LUM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-338
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This LUM protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- LUM (Human) Recombinant Protein (P01)
- Sequence
- MSLSAFTLFL ALIGGTSGQY YDYDFPLSIY GQSSPNCAPE CNCPESYPSA MYCDELKLKS VPMVPPGIKY LYLRNNQIDH IDEKAFENVT DLQWLILDHN LLENSKIKGR VFSKLKQLKK LHINHNNLTE SVGPLPKSLE DLQLTHNKIT KLGSFEGLVN LTFIHLQHNR LKEDAVSAAF KGLKSLEYLD LSFNQIARLP SGLPVSLLTL YLDNNKISNI PDEYFKRFNA LQYLRLSHNE LADSGIPGNS FNVSSLVELD LSYNKLKNIP TVNENLENYY LEVNQLEKFD IKSFCKILGP LSYSKIKHLR LDGNRISETS LPPDMYECLR VANEVTLN
- Characteristics
- Human LUM full-length ORF ( NP_002336.1, 1 a.a. - 338 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Lumican (LUM) protein (His tag)
LUM Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Lumican (LUM) (AA 15-338) protein (His tag)LUM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lumican (LUM) (AA 19-338) protein (His tag)Crystallography grade LUM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lumican (LUM) (AA 19-338) protein (His tag)Crystallography grade LUM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lumican (LUM) proteinLUM Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Fibrinogen, riboflavin, and UVA to immobilize a corneal flap--molecular mechanisms." in: Investigative ophthalmology & visual science, Vol. 53, Issue 10, pp. 5991-6003, (2012) (PubMed).
: "Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13011-22, (2011) (PubMed).
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: "Fibrinogen, riboflavin, and UVA to immobilize a corneal flap--molecular mechanisms." in: Investigative ophthalmology & visual science, Vol. 53, Issue 10, pp. 5991-6003, (2012) (PubMed).
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- Target
- LUM (Lumican (LUM))
- Alternative Name
- LUM (LUM Products)
- Synonyms
- MGC64527 Protein, zgc:86661 Protein, ldc Protein, slrr2d Protein, DKFZp468L1116 Protein, LDC Protein, SLRR2D Protein, Ldc Protein, lumican Protein, lumican L homeolog Protein, LUM Protein, lum.L Protein, lum Protein, CpipJ_CPIJ010209 Protein, Lum Protein
- Background
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Full Gene Name: lumican
Synonyms: LDC,SLRR2D - Gene ID
- 4060
- NCBI Accession
- NM_002345
- Pathways
- Glycosaminoglycan Metabolic Process
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