MOB1A Protein (AA 2-216) (His tag)

Catalog No. ABIN1097164

This Recombinant MOB1A protein is expressed in Escherichia coli (E. coli).

Quick Overview for MOB1A Protein (AA 2-216) (His tag) (ABIN1097164)

Target: MOB1A (MOBKL1B) (MOB1, Mps One Binder Kinase Activator-Like 1B (MOBKL1B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-216

Purification tag / Conjugate: This MOB1A protein is labelled with His tag.

Purpose: Recombinant Human MOB Kinase Activator 1A/MOB1A (C-6His)

Sequence: SFLFSSRSSK TFKPKKNIPE GSHQYELLKH AEATLGSGNL RQAVMLPEGE DLNEWIAVNT VDFFNQINML YGTITEFCTE ASCPVMSAGP RYEYHWADGT NIKKPIKCSA PKYIDYLMTW VQDQLDDETL FPSKIGVPFP KNFMSVAKTI LKRLFRVYAH IYHQHFDSVM QLQEEAHLNT SFKHFIFFVQ EFNLIDRREL APLQELIEKL GSKDRLEHHH HHH

Characteristics:

Recombinant Human MOB Kinase Activator 1A/MOB1A (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.15M NaCl, pH 8.0 .

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MOB1A (MOBKL1B) (MOB1, Mps One Binder Kinase Activator-Like 1B (MOBKL1B))

Alternative Name: MOB Kinase Activator 1A/MOB1A

Background: Recombinant Human MOB Kinase Activator 1A/MOB1A is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Arg216) of Human MOB1A fused with a 6His tag at the C-terminus.
MOB Kinase Activator 1A (MOB1A) belongs to the MOB1/phocein family, which acts as an activator of LATS1/2 in the Hippo signaling pathway, plays a key role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MOBKL1B stimulates the kinase activity of STK38 and STK38L. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase.

Molecular Weight: 26 kDa

UniProt: Q9H8S9