MOBKL3 Protein (AA 1-225) (His tag)

Catalog No. ABIN1097167

This Recombinant MOBKL3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for MOBKL3 Protein (AA 1-225) (His tag) (ABIN1097167)

Target: MOBKL3 (MOB Family Member 4, Phocein (MOBKL3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-225

Purification tag / Conjugate: This MOBKL3 protein is labelled with His tag.

Purpose: Recombinant Human MOB-Like Protein Phocein/MOB4/MOBKL3 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD CSNIDKILEP PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM TATEQWIFLC AAHKTPKECP AIDYTRHTLD GAACLLNSNK YFPSRVSIKE SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE YENETFLCHR FTKFVMKYNL MSKDNLIVPI LEEEVQNSVS GESEA

Characteristics: Recombinant Human MOB-Like Protein Phocein/MOB4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala225) of Human MOB4 fused with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: MOBKL3 (MOB Family Member 4, Phocein (MOBKL3))

Alternative Name: MOB-Like Protein Phocein/MOB4

Sub Type: Fusionprotein

Background: MOB-Like Protein Phocein is a member of the MOB1/Phocein Family. MOB-Like Protein Phocein is associated with membranes and the Golgi stacks. It is present in the cytosol, where it behaves as a protein complex. It has been shown that MOB-Like Protein Phocein interacts with DNM1, EPS15 and Nucleoside Diphosphate Kinase. MOB-Like Protein Phocein is the major partner of Striatin Family members and may play a important role in membrane trafficking, specifically in membrane budding reactions.
Alternative Names: MOB-Like Protein Phocein, 2C4D, Class II mMOB1, Mob1 Homolog 3, Mob3, Mps One Binder Kinase Activator-Like 3, Preimplantation Protein 3, MOB4, MOB3, MOBKL3, PHOCN, PREI3

Molecular Weight: 28.2 kDa

UniProt: Q9Y3A3