NHP2L1 Protein (AA 2-128) (His tag)

Catalog No. ABIN1097233

Recombinant NHP2L1 protein expressed in Escherichia coli (E. coli).

Quick Overview for NHP2L1 Protein (AA 2-128) (His tag) (ABIN1097233)

Target: NHP2L1 (NHP2 Non-Histone Chromosome Protein 2-Like 1 (NHP2L1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-128

Purification tag / Conjugate: This NHP2L1 protein is labelled with His tag.

Purpose: Recombinant Human NHP2-Like Protein 1/NHP2L1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MTEADVNPKA YPLADAHLTK KLLDLVQQSC NYKQLRKGAN EATKTLNRGI SEFIVMAADA EPLEIILHLP LLCEDKNVPY VFVRSKQALG RACGVSRPVI ACSVTIKEGS QLKQQIQSIQ QSIERLLV

Characteristics: Recombinant Human NHP2-Like Protein 1/NHP2L1 is produced by our E. coli expression system. The target protein is expressed with sequence (Thr2-Val128) of Human NHP2L1 fused with a His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 600 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: NHP2L1 (NHP2 Non-Histone Chromosome Protein 2-Like 1 (NHP2L1))

Alternative Name: NHP2-like-protein-1

Sub Type: Fusionprotein

Background: NHP2-Like Protein 1 (NHP2L1) is a member of the ribosomal protein L7Ae family. NHP2L1 protein is limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 has been shown to interact with RAD17and PRPF31. The protein undergoes a conformational change upon RNA-binding. NHP2L1 binds to the 5-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly, prior to step I of splicing catalysis.
Alternative Names: NHP2-Like Protein 1, High Mobility Group-Like Nuclear Protein 2 Homolog 1, OTK27, SNU13 Homolog, hSNU13, U4/U6.U5 tri-snRNP 15.5 kDa Protein, NHP2L1

Molecular Weight: 16.3 kDa

UniProt: P55769