PAFAH1B2 Protein (AA 2-229) (His tag)

Catalog No. ABIN1097287

This Recombinant PAFAH1B2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for PAFAH1B2 Protein (AA 2-229) (His tag) (ABIN1097287)

Target: PAFAH1B2 (Platelet-Activating Factor Acetylhydrolase 1b, Catalytic Subunit 2 (30kDa) (PAFAH1B2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-229

Purification tag / Conjugate: This PAFAH1B2 protein is labelled with His tag.

Purpose: Recombinant Human PAF-AH subunit β/PAFAHB (C-6His)

Sequence: SQGDSNPAAI PHAAEDIQGD DRWMSQHNRF VLDCKDKEPD VLFVGDSMVQ LMQQYEIWRE LFSPLHALNF GIGGDTTRHV LWRLKNGELE NIKPKVIVVW VGTNNHENTA EEVAGGIEAI VQLINTRQPQ AKIIVLGLLP RGEKPNPLRQ KNAKVNQLLK VSLPKLANVQ LLDTDGGFVH SDGAISCHDM FDFLHLTGGG YAKICKPLHE LIMQLLEETP EEKQTTIAVE HHHHHH

Characteristics:

Recombinant Human PAF-AH subunit β/PAFAHB (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PAFAH1B2 (Platelet-Activating Factor Acetylhydrolase 1b, Catalytic Subunit 2 (30kDa) (PAFAH1B2))

Alternative Name: Platelet-Activating Factor Acetylhydrolase IB Subunit beta/pafahb

Background: Recombinant Human Platelet-Activating Factor Acetylhydrolase IB Subunit β/PAFAHB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala229) of Human PAFAHB fused with a His tag at the C-terminus.
Platelet-Activating Factor Acetylhydrolase IB Subunit β (PAFAHB) is a cytoplasmic hydrolase. PAFAHB is a member of the GDSL lipolytic enzyme family. It also belongs to Platelet-activating factor acetylhydrolase IB β/γ subunits subfamily. Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (α), 30 kDa (β) and 29 kDa (γ), PAFAHB is a catalytic subunit. PAFAHB inactivates PAF by removing the acetyl group at the sn-2 position.

Molecular Weight: 26.6 kDa

UniProt: P68402