PDILT Protein (AA 21-584) (His tag)

Catalog No. ABIN1097311

Recombinant PDILT protein expressed in Human Cells.

Quick Overview for PDILT Protein (AA 21-584) (His tag) (ABIN1097311)

Target: PDILT (Protein Disulfide Isomerase-Like, Testis Expressed (PDILT))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-584

Purification tag / Conjugate: This PDILT protein is labelled with His tag.

Purpose: Recombinant Human Protein Disulfide-Isomerase-Like Protein of the Testis/PDILT (C-6His)

Sequence: SPEVNAGVSS IHITKPVHIL EERSLLVLTP AGLTQMLNQT RFLMVLFHNP SSKQSRNLAE ELGKAVEIMG KGKNGIGFGK VDITIEKELQ QEFGITKAPE LKLFFEGNRS EPISCKGVVE SAALVVWLRR QISQKAFLFN SSEQVAEFVI SRPLVIVGFF QDLEEEVAEL FYDVIKDFPE LTFGVITIGN VIGRFHVTLD SVLVFKKGKI VNRQKLINDS TNKQELNRVI KQHLTDFVIE YNTENKDLIS ELHIMSHMLL FVSKSSESYG IIIQHYKLAS KEFQNKILFI LVDADEPRNG RVFKYFRVTE VDIPSVQILN LSSDARYKMP SDDITYESLK KFGRSFLSKN ATKHQSSEEI PKYWDQGLVK QLVGKNFNVV VFDKEKDVFV MFYAPWSKKC KMLFPLLEEL GRKYQNHSTI IIAKIDVTAN DIQLMYLDRY PFFRLFPSGS QQAVLYKGEH TLKGFSDFLE SHIKTKIEDE DELLSVEQNE VIEEEVLAEE KEVPMMRKGL PEQQSPELEN MTKYVSKLEE PAGKKKTSEE VVVVVAKPKG PPVQKKKPKV KEELVDHHHH HH

Characteristics: Recombinant Human Protein Disulfide-Isomerase-Like Protein of the Testis/PDILT produced by transfected human cells is a secreted protein with sequence (Ser21-Leu584) of Human PDILT fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PDILT (Protein Disulfide Isomerase-Like, Testis Expressed (PDILT))

Alternative Name: pdilt

Sub Type: Fusionprotein

Background: Protein Disulfide-Isomerase-Like Protein of the Testis (PDILT) is a protein that belongs to the protein disulfide isomerase family. Human PDILT is synthesized as a 584 amino acid precursor that contains an 20 amino acid signal sequence and a 564 amino acid mature chain. PDILT contains 1 thioredoxin domain lacks the conserved redox-active Cys at position 417 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity. PDILT is an enzyme in the endoplasmic reticulum in eukaryotes. It is not a disulfide-linked homodimer. The PDILT protein can interacts with ERO1L and CLGN. PDILT probable redox-inactive chaperone involved in spermatogenesis.
Alternative Names: Protein Disulfide-Isomerase-Like Protein of the Testis, PDILT

Molecular Weight: 65.54 kDa

UniProt: Q8N807

Pathways: Cell RedoxHomeostasis