APCS Protein (AA 20-223) (His tag)

Catalog No. ABIN1097317

Recombinant APCS protein expressed in Human Cells.

Quick Overview for APCS Protein (AA 20-223) (His tag) (ABIN1097317)

Target: APCS (Amyloid P Component, Serum (APCS))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 20-223

Purification tag / Conjugate: This APCS protein is labelled with His tag.

Purpose: Recombinant Human Serum Amyloid P-Component/Pentraxin 2/SAP (C-6His)

Sequence: HTDLSGKVFV FPRESVTDHV NLITPLEKPL QNFTLCFRAY SDLSRAYSLF SYNTQGRDNE LLVYKERVGE YSLYIGRHKV TSKVIEKFPA PVHICVSWES SSGIAEFWIN GTPLVKKGLR QGYFVEAQPK IVLGQEQDSY GGKFDRSQSF VGEIGDLYMW DSVLPPENIL SAYQGTPLPA NILDWQALNY EIRGYVIIKP LVWVDHHHHH H

Characteristics: Recombinant Human Serum Amyloid P-Component/SAP produced by transfected human cells is a secreted protein with sequence (His20-Val223) of human APCS fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: APCS (Amyloid P Component, Serum (APCS))

Sub Type: Fusionprotein

Background: Serum Amyoid P Component (SAP) is a monomeric 25 kDa secreted serum glycoprotein that belongs to the pentraxins family. The members of pentaxin superfamily be characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. SAP is a non-fibrillar component, it can interact with DNA and histones. It regulates the solubility of amyloid fibrils and protects them from degradation by proteolytic enzymes and phagocytic cells. SAP scavenge nuclear material released from damaged circulating cells. It has been proposed that SAP may function as an opsonin for a variety of ligands including autoantigens, apoptotic cells, chromatin and micro-organisms.
Alternative Names: Serum Amyloid P-Component, SAP, 9.5S Alpha-1-Glycoprotein, APCS, PTX2

Molecular Weight: 24.2 kDa

UniProt: P02743