PKC epsilon Protein (AA 580-737) (His tag)

Catalog No. ABIN1097338

This Recombinant PKC epsilon protein is produced in Escherichia coli (E. coli).

Quick Overview for PKC epsilon Protein (AA 580-737) (His tag) (ABIN1097338)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 580-737

Purification tag / Conjugate: This PKC epsilon protein is labelled with His tag.

Purpose: Recombinant Human Protein Kinase C Type ε/PKC ε/PKCE (C-6His)

Sequence: MQELEYGPSV DWWALGVLMY EMMAGQPPFE ADNEDDLFES ILHDDVLYPV WLSKEAVSIL KAFMTKNPHK RLGCVASQNG EDAIKQHPFF KEIDWVLLEQ KKIKPPFKPR IKTKRDVNNF DQDFTREEPV LTLVDEAIVK QINQEEFKGF SYFGEDLMPL EHHHHHH

Characteristics: Recombinant Human PKC epsilon is produced with our E. coli expression system. The target protein is expressed with sequence (Gln580-Pro737) of Human PRKCE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PKC epsilon

Background: Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain, one C2 domain, one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters, a class of tumor promoters.ReferencesZhang KL, et al. Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas.PMID: 24012640http://www.ncbi.nlm.nih.gov/pubmed/24012640
Alternative Names: Protein Kinase C Epsilon Type, nPKC-Epsilon, PRKCE, PKCE

Molecular Weight: 19.64 kDa

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling