PKC epsilon Protein (AA 580-737) (His-IF2DI Tag)
Quick Overview for PKC epsilon Protein (AA 580-737) (His-IF2DI Tag) (ABIN7124653)
Target
See all PKC epsilon (PRKCE) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 580-737
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Purification tag / Conjugate
- This PKC epsilon protein is labelled with His-IF2DI Tag.
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Purpose
- Recombinant Human PRKCE
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Sterility
- 0.2 μm filtered
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Protein Kinase C, epsilon (PRKCE) (AA 1-737) protein (His tag)
custom-made PRKCE Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase C, epsilon (PRKCE) (AA 1-737) protein (His tag)custom-made PRKCE Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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Alternative Name
- PRKCE
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Background
- KPCE_HUMAN, MGC125656, MGC125657, nPKC epsilon, nPKC-epsilon, PKCE, Pkcea, PRKCE, Protein kinase C epsilon, Protein kinase C epsilon type
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Molecular Weight
- 17.3 kDa
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UniProt
- Q02156
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Pathways
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
Target
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