Podoplanin Protein (PDPN) (AA 23-131) (His tag)

Catalog No. ABIN1097363

This Recombinant Podoplanin protein is expressed in Human Cells.

Quick Overview for Podoplanin Protein (PDPN) (AA 23-131) (His tag) (ABIN1097363)

Target: Podoplanin (PDPN)

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 23-131

Purification tag / Conjugate: This Podoplanin protein is labelled with His tag.

Purpose: Recombinant Human Podoplanin/PDPN/Aggrus (C-6His)

Sequence: ASTGQPEDDT ETTGLEGGVA MPGAEDDVVT PGTSEDRYKS GLTTLVATSV NSVTGIRIED LPTSESTVHA QEQSPSATAS NVATSHSTEK VDGDTQTTVE KDGLSTVTLV DHHHHHH

Characteristics: Recombinant Human Podoplanin/PDPN produced by transfected human cells is a secreted protein with sequence (Ala23-Thr131) of Human PDPN fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Podoplanin (PDPN)

Alternative Name: podoplanin

Sub Type: Fusionprotein

Background: Podoplanin is a type-1 transmembrane protein that belongs to Podoplanin family. PDPN expressed in various specialized cell types throughout the body. It highly expressed in placenta, lung, skeletal muscle and brain, weakly expressed in brain, kidney and liver. In placenta, PDPN expressed on the apical plasma membrane of endothelium, in lung, expressed in alveolar epithelium. PDPN physiological function is related to its mucin-type character. PDPN may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, and major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It requires for normal lung cell proliferation and alveolus formation at birth and Induces platelet aggregation. Nevertheless, it doesn’t have any effect on amino acid transport and the aquaporin-type water channels.
Alternative Names: Podoplanin, Aggrus, Glycoprotein 36, Gp36, PA2.26 Antigen, T1-Alpha, T1A, PDPN, GP36

Molecular Weight: 12.16 kDa

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport