PFDN2 Protein (AA 1-154) (His tag)
Quick Overview for PFDN2 Protein (AA 1-154) (His tag) (ABIN1097397)
Target
See all PFDN2 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-154
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Purification tag / Conjugate
- This PFDN2 protein is labelled with His tag.
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Purpose
- Recombinant Human Prefoldin Subunit 2/PFDN2 (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME LNEHSLVIDT LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI IETLTQQLQA KGKELNEFRE KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS
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Characteristics
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Recombinant Human Prefoldin Subunit 2/PFDN2 (N-6His)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Prefoldin Subunit 2 (PFDN2) (AA 1-154) protein (His tag)
custom-made PFDN2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prefoldin Subunit 2 (PFDN2) (AA 1-154) protein (His tag)custom-made PFDN2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prefoldin Subunit 2 (PFDN2) (AA 1-154) protein (GST tag)PFDN2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prefoldin Subunit 2 (PFDN2) protein (Myc-DYKDDDDK Tag)PFDN2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM NaCl, 1 mM DTT, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PFDN2 (Prefoldin Subunit 2 (PFDN2))
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Alternative Name
- Prefoldin Subunit 2/PFDN2
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Background
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Recombinant Human Prefoldin Subunit 2/PFDN2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser143) of Human PFDN2 fused with a His tag at the N-terminus.
Prefoldin Subunit 2 (PFDN2) belongs to the Prefoldin β subunit family. The PFDN2 protein is one of six subunits of Prefoldin that act as a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides allowing them to fold correctly. PFDN2 binds specifically to Cytosolic Chaperonin (c-CPN) and transfers target proteins to it. PFDN2 also binds to a nascent polypeptide chain and promotes folding in settings where there are many competing pathways for non-native proteins. -
Molecular Weight
- 18.8 kDa
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UniProt
- Q9UHV9
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Pathways
- Unfolded Protein Response
Target
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