Nerve Growth Factor Protein (NGF) (AA 19-241)

Catalog No. ABIN1097406

This Recombinant Nerve Growth Factor protein is expressed in Escherichia coli (E. coli).

Quick Overview for Nerve Growth Factor Protein (NGF) (AA 19-241) (ABIN1097406)

Target: Nerve Growth Factor (NGF)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-241

Purpose: Recombinant Human pro-Nerve Growth Factor/pro-NGF (Glu19-Ala241)

Sequence: MEPHSESNVP AGHTIPQAHW TKLQHSLDTA LRRARSAPAA AIAARVAGQT RNITVDPRLF KKRRLRSPRV LFSTQPPREA ADTQDLDFEV GGAAPFNRTH RSKRSSSHPI FHRGEFSVCD SVSVWVGDKT TATDIKGKEV MVLGEVNINN SVFKQYFFET KCRDPNPVDS GCRGIDSKHW NSYCTTTHTF VKALTMDGKQ AAWRFIRIDT ACVCVLSRKA VRRA

Characteristics: Recombinant Human precursor form of Nerve Growth Factor/pro-NGF produced in E. coli is a non-glycosylated non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met with a molecular mass of 25kDa.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Nerve Growth Factor (NGF)

Background: The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.
Alternative Names: Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB

Molecular Weight: 25 kDa

UniProt: P01138

Pathways: Regulation of Cell Size