PITPNA Protein (AA 1-270) (His tag)

Catalog No. ABIN1097449

This Recombinant PITPNA protein is produced in Escherichia coli (E. coli).

Quick Overview for PITPNA Protein (AA 1-270) (His tag) (ABIN1097449)

Target: PITPNA (Phosphatidylinositol Transfer Protein, alpha (PITPNA))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-270

Purification tag / Conjugate: This PITPNA protein is labelled with His tag.

Purpose: Recombinant Human Phosphatidylinositol Transfer Protein α Isoform/PITPNA (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK DGEKGQYTHK IYHLQSKVPT FVRMLAPEGA LNIHEKAWNA YPYCRTVITN EYMKEDFLIK IETWHKPDLG TQENVHKLEP EAWKHVEAVY IDIADRSQVL SKDYKAEEDP AKFKSIKTGR GPLGPNWKQE LVNQKDCPYM CAYKLVTVKF KWWGLQNKVE NFIHKQERRL FTNFHRQLFC WLDKWVDLTM DDIRRMEEET KRQLDEMRQK DPVKGMTADD

Characteristics:

Recombinant Human Phosphatidylinositol Transfer Protein α Isoform/PITPNA (N-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM EDTA, 1 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PITPNA (Phosphatidylinositol Transfer Protein, alpha (PITPNA))

Alternative Name: Phosphatidylinositol Transfer Protein alpha Isoform/PITPNA

Background: Recombinant Human Phosphatidylinositol Transfer Protein α Isoform/PITPNA produced by our E. coli expression system. The target protein is expressed with sequence (Val2-Asp270) of Human PITPNA fused with a 6His tag at the N-terminus.
Phosphatidylinositol Transfer Protein α Isoform (PITPNA) is found in the cytoplasm and belongs to the PtdIns transfer protein family. PITPNA is a ubiquitous and highly conserved protein in multicellular eukaryotes that catalyzes the exchange of phospholipids between membranes and participates in cellular phospholipid metabolism, signal transduction and vesicular trafficking in vivo. It is expressed in a wide range of tissues and implicated in phospholipase C signaling and in the production of phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) by phosphoinositide-3-kinase.

Molecular Weight: 34 kDa

UniProt: Q00169