RNASE1 Protein (AA 29-156) (His tag)

Catalog No. ABIN1097467

This Recombinant RNASE1 protein is produced in Human Cells.

Quick Overview for RNASE1 Protein (AA 29-156) (His tag) (ABIN1097467)

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 29-156

Purification tag / Conjugate: This RNASE1 protein is labelled with His tag.

Purpose: Recombinant Human Ribonuclease Pancreatic/RNASE1 (C-6His)

Sequence: KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDSTVD HHHHHH

Characteristics:

Recombinant Human Ribonuclease Pancreatic/RNASE1 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 10 % Glycerol, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Alternative Name: Ribonuclease Pancreatic/RNASE1

Background: Recombinant Human Ribonuclease Pancreatic/RNASE1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Lys29-Thr156) of Human RNASE1 fused with a 6His tag at the C-terminus.
Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA.

Molecular Weight: 15.6 kDa

UniProt: P07998