NAPA Protein (full length) (His tag)
Quick Overview for NAPA Protein (full length) (His tag) (ABIN1097553)
Target
See all NAPA ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- full length
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Purification tag / Conjugate
- This NAPA protein is labelled with His tag.
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Purpose
- Recombinant Human α-Soluble NSF Attachment Protein/SNAP-α/NAPA (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
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Characteristics
- Recombinant Human alpha-Soluble NSF Attachment Protein/SNAP-alpha produced by E.coli expression system.Target protein is expressed with sequence (Met1-Arg295) of Human SNAPalpha (Uniprot #P54920) fused with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (His tag)
custom-made NAPA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (His tag)custom-made NAPA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) protein (GST tag)NAPA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) protein (Myc-DYKDDDDK Tag)NAPA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
Expiry Date
- 3 months
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- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
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Alternative Name
- SNAPalpha
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Sub Type
- Fusionprotein
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Background
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Alpha-Soluble NSF Attachment Protein (SNAP-alpha) is a member of the SNAP (Soluble NSF Attachment Protein) family. SNAP-alpha interacts with PRKCABP and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. SNAP-alpha is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. SNAP-alpha is in charge of the binding of NSF and therefore the formation of a 20S fusion particle.
Alternative Names: Alpha-Soluble NSF Attachment Protein, SNAP-Alpha, N-Ethylmaleimide-Sensitive Factor Attachment Protein Alpha, NAPA, SNAPA -
Molecular Weight
- 35.4 kDa
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UniProt
- P54920
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Pathways
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
Target
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