GALE Protein (AA 1-348) (His tag)
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- Target See all GALE Proteins
- GALE (UDP-Galactose-4-Epimerase (GALE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-348
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GALE protein is labelled with His tag.
- Purpose
- Recombinant Human UDP-Glucose 4-Epimerase/GALE (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA
- Characteristics
- Recombinant Human UDP-Glucose 4-Epimerase/GALE is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala348) of Human GALE fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GALE Protein
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UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (His tag)
custom-made GALE Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
UDP-Galactose-4-Epimerase (GALE) (AA 1-347) protein (His tag)custom-made GALE Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (His tag)GALE Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
UDP-Galactose-4-Epimerase (GALE) (AA 1-348) protein (GST tag)GALE Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
UDP-Galactose-4-Epimerase (GALE) (AA 1-348), (full length) protein (His-SUMO Tag)GALE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 2 mM DTT, 1 mM EDTA, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- GALE (UDP-Galactose-4-Epimerase (GALE))
- Alternative Name
- UDP-Glucose 4-Epimerase/GALE (GALE Products)
- Sub Type
- Fusionprotein
- Background
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The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.
Alternative Names: UDP-Glucose 4-Epimerase, Galactowaldenase, UDP-Galactose 4-Epimerase, GALE - Molecular Weight
- 40.44 kDa
- UniProt
- Q14376
- Pathways
- Response to Water Deprivation, Cellular Glucan Metabolic Process
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