Pallidin Protein (AA 1-172) (His tag)
Quick Overview for Pallidin Protein (AA 1-172) (His tag) (ABIN1098241)
Target
See all Pallidin (PLDN) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-172
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Purification tag / Conjugate
- This Pallidin protein is labelled with His tag.
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Sequence
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MSVPGPSSPD GALTRPPYCL EAGEPTPGLS DTSPDEGLIE DLTIEDKAVE QLAEGLLSHY LPDLQRSKQA LQELTQNQVV LLDTLEQEIS KFKECHSMLD INALFAEAKH YHAKLVNIRK EMLMLHEKTS KLKKRALKLQ QKRQKEELER EQQREKEFER EKQLTARPAK RM -
Purification
- > 90% by SDS-PAGE
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Pallidin Homolog (PLDN) (AA 1-172) protein (His tag)
custom-made PLDN Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pallidin Homolog (PLDN) (AA 1-172) protein (His tag)custom-made PLDN Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pallidin Homolog (PLDN) (AA 1-172) protein (GST tag)PLDN Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pallidin Homolog (PLDN) protein (Myc-DYKDDDDK Tag)PLDN Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 2 mM DTT, 10 % glycerol, 100 mM NaCl
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Pallidin (PLDN) (Pallidin Homolog (PLDN))
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Alternative Name
- PLDN
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Background
- PLDN (Pallidin) may play a role in intracellular vesicle trafficking. It interacts with Syntaxin 13 which mediates intracellular membrane fusion. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. This protein involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. PLDN has been shown to interact with BLOC1S1, STX12, Dysbindin, CNO, BLOC1S2, MuTED and SNAPAP. Recombinant human PLDN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 21.9 kDa (192aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
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NCBI Accession
- NP_036520
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Pathways
- Synaptic Vesicle Exocytosis
Target
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