ESAT6 (AA 1-95) protein (His tag)

Catalog No. ABIN7676528

This Recombinant protein is produced in Baculovirus infected Insect Cells.

Quick Overview for ESAT6 (AA 1-95) protein (His tag) (ABIN7676528)

Target: ESAT6

Protein Type: Recombinant

Origin: Mycobacterium tuberculosis

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-95

Purification tag / Conjugate: His tag

Sequence: MTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFA

Purification: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: Early secretory antigenic target, 6 kDa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ESAT6

Background: ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Molecular Weight: 11 kDa (104aa)

NCBI Accession: NP_338543