Antigen 85-A (AA 44-338) protein (His tag)
This Recombinant protein is produced in Baculovirus infected Insect Cells.
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Quick Overview for Antigen 85-A (AA 44-338) protein (His tag) (ABIN1098251)
Target
Antigen 85-A
Protein Type
Recombinant
Origin
Mycobacterium tuberculosis
Source
Baculovirus infected Insect Cells
Application
SDS-PAGE (SDS)
Purity
> 95% by SDS-PAGE
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Protein Characteristics
- AA 44-338
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Purification tag / Conjugate
- His tag
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Sequence
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FSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGA -
Purification
- purified by chromatography
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Antigen 85-A (AA 53-331), (partial) protein (His tag)
Origin: Mycobacterium tuberculosis Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Antigen 85-A
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Alternative Name
- Antigen 85A
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Background
- Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6, 6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 32.8 kDa (305aa)
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NCBI Accession
- NP_218321
Target
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