GPN1 Protein (AA 1-374) (His tag)

Catalog No. ABIN1098319

Recombinant GPN1 protein expressed in Escherichia coli (E. coli).

Quick Overview for GPN1 Protein (AA 1-374) (His tag) (ABIN1098319)

Target: GPN1 (GPN-Loop GTPase 1 (GPN1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-374

Purification tag / Conjugate: This GPN1 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAASAA AAELQASGGP RHPVCLLVLG MAGSGKTTFV QRLTGHLHAQ GTPPYVINLD PAVHEVPFPA NIDIRDTVKY KEVMKQYGLG PNGGIVTSLN LFATRFDQVM KFIEKAQNMS KYVLIDTPGQ IEVFTWSASG TIITEALASS FPTVVIYVMD TSRSTNPVTF MSNMLYACSI LYKTKLPFIV VMNKTDIIDH SFAVEWMQDF EAFQDALNQE TTYVSNLTRS MSLVLDEFYS SLRVVGVSAV LGTGLDELFV QVTSAAEEYE REYRPEYERL KKSLANAESQ QQREQLERLR KDMGSVALDA GTAKDSLSPV LHPSDLILTR GTLDEEDEEA DSDTDDIDHR VTEESHEEPA FQNFMQESMA QYWKRNNK

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: XPA binding protein 1, GTPase, GPN1, ATPBD1A, MBDIN, NTPBP

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT, 50 mM NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GPN1 (GPN-Loop GTPase 1 (GPN1))

Alternative Name: XAB1

Background: XAB1, as known as GPN1, belongs to the GPN-loop GTPase family. Small GTPases share a biochemical mechanism and act as binary molecular switches. One important function of small GTPases in the cell is nucleocytoplasmic transport of both proteins and RNA. This protein may play a role in DNA repair and may function in activation of transcription. And it forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Recombinant human XAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 44.3 kDa (398aa) confirmed by MALDI-TOF

NCBI Accession: NP_009197