POLR2J3 Protein (AA 1-115) (His tag)

Catalog No. ABIN7676579

This Recombinant POLR2J3 protein is produced in Escherichia coli (E. coli).

Quick Overview for POLR2J3 Protein (AA 1-115) (His tag) (ABIN7676579)

Target: POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-115

Purification tag / Conjugate: This POLR2J3 protein is labelled with His tag.

Sequence: MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 1 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))

Alternative Name: POLR2J3

Background: DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 15.5 kDa (138aa) confirmed by MALDI-TOF

NCBI Accession: NP_001091084