C2orf60 Protein (AA 1-315) (His tag)
Quick Overview for C2orf60 Protein (AA 1-315) (His tag) (ABIN1098482)
Target
See all C2orf60 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-315
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Purification tag / Conjugate
- This C2orf60 protein is labelled with His tag.
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Sequence
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MAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE -
Purification
- > 95% by SDS-PAGE
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Chromosome 2 Open Reading Frame 60 (C2orf60) (AA 1-315) protein (His tag)
custom-made C2orf60 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Chromosome 2 Open Reading Frame 60 (C2orf60) proteinC2orf60 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
Chromosome 2 Open Reading Frame 60 (C2orf60) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)C2orf60 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Synonyms: tRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- C2orf60 (Chromosome 2 Open Reading Frame 60 (C2orf60))
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Alternative Name
- TYW5
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Background
- TRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 38.9 kDa (338aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001034782
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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