Hemagglutinin Protein (HA) (AA 17-338) (His tag)

Catalog No. ABIN1098607

Recombinant Hemagglutinin protein expressed in Baculovirus infected Insect Cells.

Quick Overview for Hemagglutinin Protein (HA) (AA 17-338) (His tag) (ABIN1098607)

Target: Hemagglutinin (HA)

Protein Type: Recombinant

Origin: Influenza A Virus H5N1

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 17-338

Virus Strain: A/Vietnam/HN31242/2007

Purification tag / Conjugate: This Hemagglutinin protein is labelled with His tag.

Sequence: DQICIG YHANNSTEQV DTIMEKNVTV THAQDILEKT HNGKLCDLDG VKPLILRDCS VAGWLLGNPM CDEFINVPEW SYIVEKANPA NDLCYPGNFN DYEELKHLLS RINHFEKIQI IPKSSWSDHE ASSGVSSACP YQGVPSFFRN VVWLIKKNNT YPTIKRSYNN TNQEDLLILW GIHHSNDAAE QTKLYQNPTT YISVGTSTLN QRLVPKIATR SKVNGQSGRM DFFWTILKPN DAINFESNGN FIAPEYAYKI VKKGDSAIMK SEVEYGNCNT KCQTPIGAIN SSMPFHNIHP LTIGECPKYV KSNKLVLATG LRNSPL

Purification: purified by using conventional chromatography.

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Hemagglutinin (HA)

Alternative Name: H5N1 Hemagglutinin/HA1

Target Type: Influenza Protein

Background: H5N1/HA (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/Viet Nam/HN31242/2007 (H5N1) ) HA1 protein, fused to His-tag at C-terminus, was expressed in insect cell using baculovirus expression system and purified by using conventional chromatography.

Molecular Weight: 37.4 kDa (332aa)