GPR177/WLS Protein (AA 101-232) (His tag)

Catalog No. ABIN1098794

This Recombinant GPR177/WLS protein is produced in Escherichia coli (E. coli).

Quick Overview for GPR177/WLS Protein (AA 101-232) (His tag) (ABIN1098794)

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 101-232

Purification tag / Conjugate: This GPR177/WLS protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Alternative Name: GPR177/WLS

Background: WLS, a Wnt trafficking regulator, is a multipass transmembrane protein that regulates the sorting and secretion of Wnt protein. It is required for organogenesis during embryogenesis and for the establishment of the anterior-posterior axis. WLS promotes various cancers such as glioma tumourigenesis. The gene encoding WLS maps to human chromosome 1 and is expressed as multiple alternatively spliced isoforms. Recombinant human WLS protein, fused to His-tag at N-terminus, was expressed in E. coli.

Molecular Weight: 17.8 kDa (155aa)

NCBI Accession: NP_079187

Pathways: WNT Signaling