CXCL10 Protein

Catalog No. ABIN1305104

This Recombinant CXCL10 protein is produced in Escherichia coli (E. coli). There are 4 publications available.

Quick Overview for CXCL10 Protein (ABIN1305104)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Biological Activity: This preparation of recombinant human IP-10 protein has not been evaluated for biological activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Characteristics: The recombinant human IP-10 was produced and found to be ≥ 95% pure by SDS-PAGE analysis and visualized by silver strain.

Purification: The recombinant human IP-10 was produced and found to be ≥ 95 % pure by SDS-PAGE analysis and visualized by silver strain.

Sterility: 0.22 μm filtered

Application Details

Application Notes: ELISA Standard: The recombinant human IP-10 (Cat. No. 551130) is useful as a quantitative standard for a sandwich ELISA for measuring human IP-10 protein levels. Purified 4D5 antibody (Cat. No. 555046) can be paired with the biotinylated 6D4 antibody (Cat. No. 555048) with recombinant human IP-10 as the standard. To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~1,000 to 5 pg/ml are recommended for inclusion in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human-IP-10 in serum or plasma our BD OptEIA™ Human IP-10 Set (Cat. No. 550926) is specially formulated and recommended.

Comment:

BD Pharmingen™ Recombinant Human IP-10

Restrictions: For Research Use only

Handling

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Recombinant human IP-10 is supplied lyophilized from a solution of aqueous buffered solution containing 50 µg BSA per 1 µg of cytokine as carrier protein. No sodium azide has been added to the preparation and the endotoxin level is less than 1 EU per 1 µg of the cytokine as determined by the LAL method. The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10.0 mg/ml carrier protein such as human or bovine serum albumin, aliquoted into polypropylene microtubes and stored at -80°C.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Recombinant human IP-10 is supplied lyophilized from a solution of aqueous buffered solution containing 50 μg BSA per 1 μg of cytokine as carrier protein. No sodium azide has been added to the preparation and the endotoxin level is less than 1 EU per 1 μg of the cytokine as determined by the LAL method. The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10.0 mg/ml carrier protein such as human or bovine serum albumin, aliquoted into polypropylene microtubes and stored at -80°C. NOTE: Failure to add carrier protein** or store at indicated temperatures may result in a loss of activity.

References

Farber: "Mig and IP-10: CXC chemokines that target lymphocytes." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 246-57, (1997) (PubMed).

Loetscher, Gerber, Loetscher, Jones, Piali, Clark-Lewis, Baggiolini, Moser: "Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 963-9, (1997) (PubMed).

Luster, Ravetch: "Biochemical characterization of a gamma interferon-inducible cytokine (IP-10)." in: The Journal of experimental medicine, Vol. 166, Issue 4, pp. 1084-97, (1987) (PubMed).

Luster, Jhanwar, Chaganti, Kersey, Ravetch: "Interferon-inducible gene maps to a chromosomal band associated with a (4;11) translocation in acute leukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 9, pp. 2868-71, (1987) (PubMed).

Target Details

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: IP-10

Background: Interferon-γ inducible protein-10 (IP-10) is a 10 kD protein that belongs to CXC chemokine family. Unlike other CXC chemokines, IP-10 is a chemoattractant for activated lymphocytes, but not resting lymphocytes or neutrophils. The gene for human IP-10 has been mapped to chromosome 4q2. CXC chemokine receptor 3 (CXCR3) has been identified as the receptor for IP-10.